The PURE-LAMP test consists of three steps, sample preparation, Amplification, and visual detection of fluorescence light from the reaction tube using UV light. Early diagnosis is important for the control and prevention of TB. With the development of molecular biology, a number of methods have been developed for the rapid detection and diagnosis of TB. LAMP is a new nucleic acid amplification technology first proposed by Notomi in 2000. The PURELAMP test is a new, simple, contamination-resistant kit for the diagnosis of TB. In this study, the sensitivity of PURE-LAMP based on spot sputum in Benzoylpaeoniflorin smear-negative and culture-positive patients was 53.81%, the overall sensitivity of PURE-LAMP was 70.67%, and the specificity of PURE-LAMP was 98.32%, which was similar with that found in other studies. Comparing the sensitivity of PURE-LAMP with that of smear microscopy, the sensitivity of PURE-LAMP was higher than the sensitivity of smear microscopy. This result showed that PURE-LAMP can be used for MTB diagnosis. Chinese national guidelines have recommended obtaining three sputum specimens from patients with suspected TB. In the present study, we assessed the contribution of each specimen collected to the ultimate diagnosis of MTB for TB suspects and found that the sensitivity of the PURE-LAMP test for MTB detection in TB suspects from three sputa was significantly higher than that in one test of spot sputum. Considering the cost factor, testing three samples may increase the economic burden of patients. Therefore, it is necessary to conduct an analysis of the cost-effectiveness of the PURE-LAMP test with different specimen combinations in the future. The contamination rate of PURE-LAMP was 0.21% in this study. Two labs in this study were both equipped with three independent and separate working areas, including the storage area for Praeruptorin-B reagents, the pretreatment area, and the amplification and results analysis area. Each room was clearly labeled to avoid confusion when retrieving equipment or materials from different areas. Work benches were disinfected by a 5% sodium hypochlorite solution and exposed to UV light after each testing. Through strict control of contamination and the 
closed system of the PURE-LAMP test, the risk of contamination was highly reduced. We also found PURE-LAMP��s reporting time much shorter than that of solid culture: a diagnosis can be completed within two hours. Fluorescence results were examined with the naked eye by lab technicians, and no indeterminate cases were found. China��s NTRL rechecked 20% of the sample results, and the concordance rate was 100%. In conclusion, the PURE-LAMP test is a new diagnostic technology that can rapidly and accurately detect TB in patients. Among various insects, silkworm moth was identified as an important inhalant allergen in several studies worldwide. In southern China where insects grow well in hot and humid climates, previous local epidemiological study in Guangzhou showed that up to 40% of patients with respiratory allergy were sensitized to silkworm moth, and the prevalence of sensitization to silkworm moth was one of leading causes of allergy, immediately ranked after house dust mites and before cockroaches.