{"id":1645,"date":"2020-03-18T16:47:48","date_gmt":"2020-03-18T08:47:48","guid":{"rendered":"http:\/\/www.bioactivescreeninglibrary.com\/?p=1645"},"modified":"2022-01-07T10:56:45","modified_gmt":"2022-01-07T02:56:45","slug":"panel-summarizes-data-report-ted-compartments-computed-considered-developmental-stages","status":"publish","type":"post","link":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/2020\/03\/18\/panel-summarizes-data-report-ted-compartments-computed-considered-developmental-stages\/","title":{"rendered":"This panel summarizes data report ted in both compartments computed for all the considered developmental stages"},"content":{"rendered":"

In the orange bars indicate the mean number of active electrodes detected in the lower compartment while the blue ones the mean number of active electrodes detected in the upper compartment, both computed by taking into account all performed experiments at each developmental time frame. It is possible to observe that the two different color bars are approximately the same height at each time frame, thus indicating a comparable number of active electrodes in both compartments with very small variability during the development. Since both compartments have roughly the same number of electrodes recording firing activity, this indicates that the presence of the physical constraint due to the PDMS mask does not prevent cells from equally distributing on the entire recording area. Before proceeding in investigating the NB dynamics, we wanted to analyze how many channels were involved in the generation of the NBs. To do so, the Major Burst Leaders have been identified. Fig. 5B reports the mean number of MBLs and the percentage of MBLs over the total number of active electrodes. The percentage of MBLs for hippocampal uniform networks is also reported on the same graph. It is possible to observe that the mean number of MBLs does not significantly change during development, and that the percentage of MBLs is upper limited by 12%. Since the number of MBLs identified per each culture is low compared to the number of its active electrodes, it is possible to conclude that there is a small group of electrodes leading the entire network activity during the development also in confined modular cultures. We then asked whether the so generated NBs preferably remained in the same compartment or propagated towards the other one. Fig. 5C shows the Network Burst Localization parameter for the seven considered modular networks. As reported in the Methods section, the NBL indicates where the different NBs mostly propagate. The graph indicates that each culture has indeed a ‘dominant’ module that remains the same during the entire development, except for two cultures which do not Tubulin Acetylation Inducer cost<\/a> present a dominant compartment, respectively at DIV 16-25 and DIV 46-55. Fig. 5D shows the percentage of experiments in which there is a clear-cut ‘NBL dominance’, with respect to the total number of performed recordings. We then decided to investigate more in detail if there is a relationship between the level of firing of each compartment and the NBL dominance. Fig. 6A shows the average MFR computed inside each compartment per each performed experiment, for the entire monitored period. We performed a t-test in order to assess whether or not there is a statistical difference between the upper and the lower compartment’s firing, for all the considered cases. Fig. 6B on the left shows the percentage of recordings in which one compartment is more active than the other, and the percentage of recordings in which both compartments have the same level of firing.<\/p>\n","protected":false},"excerpt":{"rendered":"

In the orange bars indicate the mean number of active electrodes detected in the lower compartment while the blue ones the mean number of active electrodes detected in the upper compartment, both computed by taking into account all performed experiments at each developmental time frame. It is possible to observe that the two different color … Continue reading “This panel summarizes data report ted in both compartments computed for all the considered developmental stages”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/1645"}],"collection":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/comments?post=1645"}],"version-history":[{"count":1,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/1645\/revisions"}],"predecessor-version":[{"id":1646,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/1645\/revisions\/1646"}],"wp:attachment":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/media?parent=1645"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/categories?post=1645"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/tags?post=1645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}