By using a Blast search of coding sequences for VP23 and VP5 in different HSV-1 strains, we found that VP23 and VP5 are highly conserved in different viruses, and even the similarities between HSV-1 and HSV-2 are greater than 87%. These findings suggest that VP5 and VP23 are necessary for HSV-1 to proliferate, opening up possibilities for VP5 and VP23 as new targets in anti-HSV therapies. The failure of the null mutant in VP19C to form detectable virions has previously been verified by electron microscopy and sedimentation analyses following infection of nonpermissive cells. Cell localization studies also demonstrated the requirement of VP19C for the proper nuclear localization of VP23. VP26 is not a component of the pre-capsid and not necessary for capsid assembly of HSV-1. Thomsen et al. and Tatman et al. have developed procedures for using recombinant baculoviruses to produce HSV-1 capsids in insect cells. They found that the minimal number of genes required for assembly of capsids is four, including the UL18, UL19 and UL38 genes and either the UL26.5 or the UL26 gene. Previous studies have shown that the products of UL26 and UL26.5 genes are scaffold proteins, which form a core internal to the capsid shell and interact directly with VP5. These interactions are essential to the assembly of to the icosahedral capsids. However, in our investigation, silencing UL26 and UL26.5 as well as VP19C did not influence HSV-1 replication. Perhaps host cells proteins or other viral proteins can partly compensate for the function of these capsid proteins, but this speculation requires further investigation. The viral genome is the most important structure of HSV and it endows the virus with the ability of pathogenicity. The process of viral DNA replication and assembly has been well studied. Viral DNA synthesis begins shortly after the appearance of the beta proteins and continues up to 15 h post-infection. Previous studies have shown that there are at least seven HSV-1 genes that are necessary for DNA replication including UL5, UL8, UL9, UL29, UL30, UL42, UL52 and many host proteins are also involved in this process. These viral proteins involved in DNA replication have provided useful targets for antiviral therapy. After the procapsid was assembled, the viral DNA was packaged in the capsid with the help of several viral proteins such as UL12 encoding protein. And this process is extremely important for the replication of virus in host cells. It determines whether the virus infection can form a complete infectious progeny virus. In the present study we find that knockdown of capsid protein VP23 and VP5 block the process of capsid formation and disturbance the process of DNA packaging. By quantifying the cellular viral genome copy numbers, we find that blocking the expression of VP23 and VP5 greatly decreased the total viral load in the cells. Forms of VP5 and VP23 failed to form plaques in cell lines.<\/p>\n","protected":false},"excerpt":{"rendered":"
By using a Blast search of coding sequences for VP23 and VP5 in different HSV-1 strains, we found that VP23 and VP5 are highly conserved in different viruses, and even the similarities between HSV-1 and HSV-2 are greater than 87%. These findings suggest that VP5 and VP23 are necessary for HSV-1 to proliferate, opening up … Continue reading “The reduction in cellular genome copy numbers should be mainly due to the inhibition of strains with mutated”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/1551"}],"collection":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/comments?post=1551"}],"version-history":[{"count":1,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/1551\/revisions"}],"predecessor-version":[{"id":1552,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/1551\/revisions\/1552"}],"wp:attachment":[{"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/media?parent=1551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/categories?post=1551"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.bioactivescreeninglibrary.com\/index.php\/wp-json\/wp\/v2\/tags?post=1551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}