Further studies are necessary in order to better understand the complex interactions between this neonatal manipulation and the reduced predisposition to developing intestinal pathologies exhibited by these animals. However, we suggest that the CORT-nursed model represents an experimental model providing new insight into the field of gastrointestinal pathologies, and demonstrating that mild neonatal stress, similar to maternal care, induces long lasting physiological changes that make the adult better adapted to colonic inflammatory stress, probably through alterations in intestinal permeability. In conclusion, the CORT-nursed model can be considered a useful tool to better explore other endogenous systems involved in individual susceptibility to colitis, in order to identify new therapeutic targets and approaches in the field of intestinal disorders. The outermost layer of the Welsh onion is composed of the cuticle and an outer epidermis of wax. The cuticular wax consists of derivatives of long-chain fatty acids, including alkanes, primary alcohols, secondary alcohols, aliphatic aldehydes, ketones and esters. The cuticular wax can protect tender leaves against insects or other abiotic stresses, including moisture loss, bacteria and herbivorous insects, and the effects of ultraviolet radiation and frost damage. The wax is mainly composed of very-long-chain fatty acids in the plant cuticle, with the fatty acids having a carbon number of 18 or higher. Such fatty acids have a wide range of physiological functions; are involved in the synthesis of seed glycerides, sphingolipids and lipid biofilms; and provide precursors for the biosynthesis of the waxy cuticle. As the waxy cuticle plays an important role in the plant’s resistance to adversity, researchers are focusing on understanding the waxy synthesis pathway, gene cloning and functional characterization. Several wax-related genes were isolated in maize and the model plant Arabidopsis; CER6, CER10, GL8A, GL8B, FDH, FAE1, KCS1 and PAS2 are involved in the synthesis of VLCFA wax precursors. CER4 and WSD1 participate in the acyl reduction pathway to catalyze the production of primary alcohol and wax ester, respectively, and are involved in the synthesis of wax components. MAH1 participates in the decarbonylation pathway to catalyze the conversion of alkanes into secondary alcohols and ketones. CER5 in Arabidopsis is the first characterized gene that encodes the plasma membranelocalized ABC transporter that is required for the transport of wax components from the epidermal cells to the cuticle. Our study demonstrates that four Welsh onion unigenes that are related to waxy cuticle synthesis function in the several processes, including long-chain fatty acid metabolism, very-long-chain fatty acid metabolism, wax biosynthesis, and oxidation-reduction; therefore, these genes may be involved in the synthesis of wax precursors. Welsh onion is a Rapamycin typical waxy plant, but the wax content and genes are not well studied. This study was designed to compare the RNA-seq results between waxy plants and non-waxy mutant plants via high-throughput sequencing technology and to identify the important genes.