This limited gene expression is often considered as one of the most important factors in the pathogenesis and escape of these malignancies from immune control, has been recognised as one of most crucial latent proteins for EBVmediated transformation of normal B cells and is uniquely able to Diacerein induce malignant outgrowth and hyperplasia in transgenic mice. Furthermore, LMP1 is also known to exhibit pleiotropic effects on the cellular phenotype of B cells which include induction of activation antigens, the expression of inhibitors of programmed cell death and NF-kB activation through the TRAF signalling pathway. Previous studies have shown that LMP1 acts as a constitutively active receptor like molecule independent of the binding of a ligand. The transmembrane domains mediate oligomerization of LMP1 molecules in the plasma membrane, a prerequisite for LMP1 function. Over the last few years, there has been increasing evidence to suggest EBV is capable of modulating the Wnt pathway. In particular, it has been suggested LMP1 expression can repress the expression of E-cadherin. The current experiments reported here were undertaken to reassess the role of LMP1 in regulating the expression of E-cadherin and to further explore the mechanism by which LMP1 modulates the Bismuth Subsalicylate function of various mediators of the canonical Wnt cascade. Here we show that transient or stable expression of LMP1 sequences from normal B cells and NPC does not impair the expression of E-Cadherin and other mediators of the Wnt pathway. Furthermore, we also demonstrate that LMP1 expression in human cells had minimal effect on the interaction of E-cadherin and b-catenin thus no evidence of b-catenin-mediated transcriptional activation was observed. To explore the effect of LMP1 on other mediators of the Wnt pathway, we transiently transfected HaCaT and MDCK cells with expression vectors encoding LMP1-GFP or the control EGFP vector. These LMP1 sequences were either derived from the prototype B95.8 isolate, spontaneous LCLs or NPC.After transfection, these cells were examined using confocal microscopy for the expression of Ecadherin, b-catenin or actin. Representative data from a series of experiments is presented in Figure 1.