In addition, the H3K4 tri-methylation states of Ehd1 were not changed in HS112. Therefore, Se14 might be indirectly involved in the repression of Ehd1 independent with the pathways of Ghd7. Further, the analysis of interallelic interaction between Se14 and Ehd1 suggested that Se14 represses 11-Ketotestosterone flowering independently of Ehd1. Thus, it is indicated that Se14 is more effective to delay flowering through the repression of RFT1 by H3K4me3 demethylation than the indirect repression of Ehd1. On the other hands, the analysis of interallelic interaction between Se14 and Se13 demonstrated that Se14 did not function in the Se13- deficient genetic background. Interestingly, the expression of Se14 was down-regulated in the Se13-deficient genetic background. Jang et al. demonstrated that the repression of phyA by light is correlated with alternations in specific histone marks. In the dark, methylation of H3K4me3 were detected at the active phyA locus, whereas upon light treatment, increased H3K27me3 mark and decreased H3K4me3 mark were associated with the repressed gene. They also reported that the chromatin modifications were blocked in the phyB-9 mutant in red light, indicating that these changes are mediated by the phyB signaling pathway. Our study also showed that the HMBA diurnal expression of Se14 was highly transcribed during the light period. It is therefore suggested that the Se14 might be mediated by the red-light signal. Further analysis is necessary to clarify the light-mediated H3K4me3 demethylation. Red-light signaling via phytochromes is the most important factor to repress flowering under LD condition. Osugi et al. showed that phytochromes not only regulate Ghd7 transcription but also affect Ghd7 protein activity. In addition to the Ghd7 protein, the Hd1 protein is known to act as a suppressor of Hd3a under long day-length conditions. Ishikawa et al. reported that the inhibitory effect of the Hd1 protein on Hd3a expression is dependent on phyB. Ishikawa et al. also suggested that the phyB-mediated Hd1 suppression of Hd3a expression is a component of the molecular mechanism for critical day length in rice. Our results have let us propose a novel flowering repression pathway mediated by red-light signaling. We believe that this pathway of Se14 will yield crucial information for understanding the daylength dependent repression of flowering in rice.