Therefore, in this study we examined two different combination of CDA; CDA combined disinfectants and CDA combined antibiotics, to introduce a promising strategy which is appropriate to control biofilms both in food industry and clinical settings. While some free fatty acids have antimicrobial properties and play a vital role in maintaining the microbial flora of the skin, we demonstrated that CDA does not inhibit bacterial growth at nano-molar ranges that induce biofilm dispersal. These results were highly in consistent with the results from Jennings et al. study where they showed that CDA inhibited bacterial growth only at high concentrations. This lack of growth inhibition at lower concentrations was not surprising since bacteria produce this unsaturated fatty acid and use it as a signalling molecule. Intervertebral discs degeneration is a significant physical condition that is frequently related to the loss of cellularity and matrix degradation in nucleus pulposus tissue, which is followed by pathological cascades and eventually causing severe discogenic low back pain. Cell therapy is highly promising to restore the cellularity, biochemistry and biofunctionality of the Chrysomycin B degenerative NP tissue. Currently, the technique is restrained by the lack of available human cell sources. Notochordal cells are the ideal for the DCHC transplantation purpose but scarcely present in adult human NP tissue. Neither autologous nor allogenic sources are available for research or future clinical applications. As a result, alternative cells have been investigated including mesenchymal stem cells and chondrocytes. Although successful in many aspects, these alternative cell sources have major limitations, such as inferior ability to produce native-like NP tissue and vulnerability to the challenging microenvironment of intervertebral discs.. In view of regenerative medicine, NCs and the terminally differentiated nucleus pulposus cells are both phenotypically correct and desirable for the purpose. NCs are more important since they can generate the NPCs and play pivot roles homeostasis of the NP tissue. Also, NCs may potentially survive better in the harsh NP microenvironement upon transplantation which are generally highly challenging for other transplanted cells. The first step to develop the therapy is to generate high-quality, functional NCs from enabling sources. HiPSCs hold great potential due to their pluripotency, abundance, and patient specificity.