In this study, central HPA axis function was only measured by basal state HPA axis tests which are generally inferior in diagnosing HPA function, while dynamic testing has the advantage of providing an assessment of stress reserve. In the future, dynamic testing will be used in evaluating the central HPA axis function of AD mice. In addition, there is a close relationship between central and skin HPA axis and it is already clear that central HPA axis in communication with cytokines can regulate local steroidogenic activity and skin immune activity, however, skin as an important peripheral neuro-endocrine-immune organ is tightly networked to central regulatory systems, the effects of skin CRH/POMC on the pituitary or adrenal functions need to be further investigated. On the other hand, Rodr��guez-Porrata et al.2 showed that the knockout mutants for four nuclear apoptotic-related genes with mitochondrial functions were hyper-tolerant of dehydration stress. Most S. cerevisiae genes Bortezomib involved in qualitative traits related to their basic biology have been identified using recombinant DNA techniques. However, many phenotypes important to industrially appear to be quantitative traits that are determined by quantitative trait loci, such as growth temperature, ethanol tolerance, acetic acid production, sporulation rate, sake aromatic compounds production, and nitrogen utilization. Considering the large amount of genetic variability in industrial yeast, a characteristic as crucial as dehydration tolerance is likely controlled by multiple QTLs that cannot be identified by conventional molecular genetic approaches. In this paper, we performed QTL analysis on 96 segregants derived from a cross between two haploid strains derivatives of two strains of wine yeast using statistical linkage analysis between dehydration tolerance characteristics and DNA marker genotype data. We functionally characterized two QTLs encompassing six genes involved in dehydration stress tolerance that contribute to the natural phenotypic variation in the paternal strains. Most of the genetic determinants of dehydration tolerance in yeast are still unknown. In this paper, two dehydration-tolerant QTLs were identified using a segregating population. By analysing strains with deleted genes in each QTL and by reciprocal hemizygosity assays, six genes have been confirmed to affect the capacity of yeast cells to survive dehydration and rehydration, namely the BUD27, FAB1, and ATG18 genes mapped to QTLs on chromosome VI and the CBT1, RSM22, and DBR1 genes in QTLs on chromosome XI. Furthermore, their phenotypic effects have been estimated. The genes ATG18, RSM22, and DBR1 were not found to be necessary for desiccation tolerance in yeast cells. The fact that the genes mapped in our results do not fully coincide with previous genetic studies carried out with the S. cerevisiae deletion libraries of mutants sensitive to dehydration stress may Perifosine abmole bioscience indicate that different cellular mechanisms for overcoming stress imposition were caused by dissimilar selective forces exerted during the evolution of the yeast strains, or because the mutations present in the laboratory strains used for these studies are the effectors of these particular phenotypes. Therefore, small discrepancies among the genes associated with cell dehydration tolerance from different studies support the idea that different allelic combinations exert different effects.