Here, we show that a less complex network consisting of a transactivator and its responsive promoter is sufficient for provoking bimodal expression in a mammalian system. In this respect, the described positive feedback modules represent ideal building blocks to artificially provoke allor-nothing-answers in mammalian cells. The mathematical model predicts a hysteretic expression pattern from the synthetic positive feedback loop. This prediction was experimentally verified. Such a strong a strong hysteretic response is expected to occur only from strong positive feedback loops. The hysteretic response of feedback modules can be tuned by the strength of the positive feedback. To achieve such a response with the synthetic modules Benazepril presented here, transactivator mutants eliciting Gelsenicine activation at different inducer levels might be of benefit. Furthermore, the hysteretic response can be modulated to different strengths through the connection of a positive feedback loop with a transcriptional repressor. Clathrin is a stable protein with a slow turnover and consequently degrading and resynthesising this protein within the timeframe of M phase is not a feasible means of disassembling and regenerating subcellular organelles and vesicles during cell cycle progression. Monochorionic multiple pregnancies complicated by twin to twin transfusion syndrome are at high risk of preterm delivery, due both to the effect of raised amniotic pressure with two or more fetuses and excessive amniotic fluid volume, and to the iatrogenic effects of therapeutic procedures. Cervical length prior to fetoscopic laser ablation has been shown to predict miscarriage and preterm labour, with values,30mm having odds ratios of 2.2�C3.5 for delivery before 28 and 34 weeks respectively. The main positive predictive value was seen in the 7% with values,20mm. However, it is unclear whether cervical shortening under these circumstances is simply an acute effect of uterine overdistension, or a more chronic process reflecting longstanding uterocervical pathology. We hypothesized that cervical shortening in complicated monochorionic twin pregnancies reflects the degree of polyhydramnios, and that normalization of amniotic fluid volume in patients with polyhydramnios leads to lengthening of the cervix.