Here, only one time point and a single concentration of the applied drugs were used to assess the blocking effects. Therefore, additional studies are needed to The expressing of P2X1 or P2X3 subunits exhibited fast activation and rapid desensitizing Polygodial currents, whereas P2X2 or P2X4 expressed alone yielded sustained ATP-activated currents. However, the PHP 501 trifluoroacetate co-expression of P2X2 and P2X3 resulted in slow, sustained IATP, whereas P2X3 and P2X4 displayed an IATP of desensitization with two components of rapid, and slow sustained IATP. In addition, the expression of P2X3 alone exhibited fast desensitizing IATP, but that the co-expression of P2X2 and P2X3 yielded mixed fast and slow desensitizing IATP. Although we did not assess the contribution of P2X5, P2X6, and P2X7 to the features of IATPs, previous studies demonstrated that the expression of cloned P2X5 resulted in a mixed desensitizing IATP with two components, whereas P2X6 exhibited slow desensitizing IATP, and P2X7 was not found in NG neurons. Boue-Grabot et al. correlated molecular structure with the function of different types of P2X receptors, and suggested that P2X1 and P2X3 formed rapid desensitizing heteromeric/homomeric receptors, whereas P2X2, P2X4, and P2X5, P2X2 and P2X3, P2X1 and P2X5, and P2X4 and P2X6 homomeric or heteromeric receptors desensitized at a low to moderate rate. By combining a whole cell patch clamp technique with in situ immunocytochemistry, we offered direct and convincing evidence that the configurations of IATPs correlated with the composition of P2X1�C4 subunits and cell size. These results also confirm previous speculation that small- and medium-sized neurons express P2X1 and/or P2X3 subunits with fast activated and rapid desensitized currents, while medium- and large-sized neurons express P2X2 or P2X4 subunits with fast activated and slowly desensitized currents. However, it should be emphasized that we obtained only limited data from single staining since different P2X subunit assemblies could not be reflected using such procedures. Therefore, double or triple staining of recorded cells should be performed for further investigation. In otherwise healthy individuals, inhalation of A. fumigatus conidia has been associated with allergic sensitization and hypersensitivity pneumonitis. In patients with chronic lung inflammatory diseases such as asthma or cystic fibrosis, inhalation of A. fumigatus can lead to allergic bronchopulmonary aspergillosis, which is marked by fungal persistence in the airways and increased inflammatory responses. However, the most severe disease occurs in neutropenic individuals or patients treated with immune suppressive drugs after hematopoietic stem cell or organ transplantation.