It is also more widely expressed in the earlier developing kidney, but again including definite podocyte expression. The Dach1 mutant mice exhibit early postnatal death, although no developmental defects were detected in any organ system examined, including kidneys . The Eya, Six and Dach encoded proteins often interact in a conserved network in development . We observed only background levels of Eya1 and Eya2 transcripts in the podocyte, and only slightly above background expression for Eya3 and Eya4. Similarly, Six1, 3, 4, 5 and 6 were present at very low levels in the podocyte, while Six2 transcripts were present at modest levels in the early developing podocyte, and essentially absent in the adult. Dach1 in the podocyte therefore appears to act independent of Six and Eya. Of particular interest, given the importance of preventing adult podocyte proliferation , DACH1 can inhibit Cyclin D1 and thereby inhibit cell proliferation . DACH1 can also inhibit JUN-mediated contact-independent growth , which could be of importance since we observe that Jun is also expressed in prodocytes. JUN is a positive regulator of cell proliferation. Of interest, loss of E-cadherin mediated cell-cell contact can upregulate Jun . Podocyte expressed transcription factors also included Hoxc4, Hoxc6, Hoxc8, Zeb1, and Mafb. Of interest, Hoxc6 and Hoxc8 are both expressed strongly in stromal cells during development, like Foxd1, as well as in forming podocytes . The targets of Hox genes can vary from cell type to cell type, but it is interesting to note that HOXC6 targets in prostate cells include elements of FGF, BMP, NOTCH and WNT signaling pathways . MAFB is a member of the Maf family of transcription factors, and has been previously shown to play a critical role in podocyte development, with mutant podocytes showing fused foot processes that did not interdigitate . Zeb1 SU5416 customer reviews encodes a zinc finger E-box binding homeobox transcription factor and has previously been associated with epithelial mesenchymal transition, in particular as a repressor of E-cadherin .