The highest scoring network for each principal component was interpreted for all metagenes, and the second highest network was considered when the difference in score between it and the top network was relatively small. S2�CS9 Figs. show all networks evaluated. Key genes were derived for each metagene from the tree by restricting to genes from the cluster where the absolute value of the loading was greater than 0.1. This value was empirically set to generate lists of the appropriate size for downstream pathway analysis without considering gene identity or function. The K-PC1 metagene was excluded from this analysis since there were only three genes in that cluster. Each gene GDC-0879 Raf inhibitor identifier was mapped to its corresponding gene object in the Ingenuity Pathways Knowledge Base. These genes were overlaid onto a global molecular network developed from information contained in the Ingenuity Pathways Knowledge Base. Networks of these focus genes were then algorithmically generated based on their connectivity. The loading of the gene relative to the metagene was used in place of a raw expression score. As a result, genes with a negative loading are positively MG132 associated with the left branch in the decision tree. Genes with a positive loading are associated with the right branch in the tree. The Functional Analysis of the top scoring network identified the biological functions and/or diseases that were most significant to the genes in the network. The network genes associated with biological functions and/or diseases in the Ingenuity Pathways Knowledge Base were considered for the analysis. Fisher��s exact test was used to calculate a p-value determining the probability that each biological function and/or disease assigned to that network is due to chance alone. CD150 receptor is a self-ligand and functions as a co-receptor molecule that regulates signaling via antigen receptors. It is also associated with several components of the bacterial killing machinery, which defines it as a novel bacterial sensor. Moreover, CD150 was found to be the major receptor for several Morbilliviruses, including measles virus, canine distemper virus and rinderpest virus. MV infection could be associated with numerous complications, of which especially severe are those that involve the central nervous system, as MV is implicated in the pathogenesis of several types of encephalitis. However, currently identified cellular receptors for wild type MV make the virus entry possible only in lymphoid cells or epithelial cells . The third MV receptor, CD46, is expressed on all human nucleated cells and is able to mediate the entry of laboratory adapted and vaccine strains of the virus.