Sequencing read depth and reduced bias should provide accurate read densities at singlenucleotide resolution, making it possible to extend our method to measure nucleotide-by-nucleotide RNA polymerization rates. Even at current sequencing depths, applying our method to assess the results of a larger set of pharmacological and genetic manipulations of pre-mRNA processing factors will likely reveal new mechanisms of pre-mRNA processing and clarify interconnections between the stages of the mRNA lifecycle. The Tubulin Acetylation Inducer prevalence of cardiovascular autonomic neuropathy is rapidly growing in all populations worldwide, particularly in the developing world. The disease is not only a major factor in the cardiovascular complications of diabetes mellitus, but also affects many other majority segments of general population, such as the elderly, patients with hypertension, metabolic syndrome, and connective tissue disorders. CAN has become a major health concern in China following rapid lifestyle changes. For example, in patients with diabetes, the prevalence of CAN was 30–60%. However, 40% of individuals with CAN were unaware of the disease, as was also found in previous studies. The age at onset of CAN seems to decrease in diabetic or/and hypertension patients. Individuals with previously undiagnosed CAN have an unfavorable cardiovascular risk profile, especially sudden death, indicating a higher risk for cardiovascular disease. Analyses of short- and long-term heart rate variability have been proven useful for detecting CA function in patients. CA function testing using HRV is sensitive, noninvasive, and reproducible; therefore, it is easily applicable for screening a large number of individuals in general population. Lifestyle modification has been proven to effectively prevent and delay the development of CAN. Delay and lack of detection of the disease was mostly resulted from patients being asymptomatic during the early stage of the disease so that a simple and accurate screening tool to identify those at high risk of developing CAN will be of great value. It was not be convenient or costeffective population screening for CAN using 24 hours Holter or Ewing’s testing, especially in a resource-poor country. In our previous studies, predictive models for CA dysfunction have been created by using artificial neural network and logistic regression approaches. Furthermore, a simple tool, using a few questions and simple measurement of anthropometric indexes, would be practical for use by the general public and in primary health care. However, a simple CAN risk score based on general Chinese population was little found. The aim of this study was to develop and evaluate a simple, noninvasive, practical, and informative scoring system to characterize individuals according to their future risk of CAN in Chinese population. A secondary aim was to evaluate the extent of improvement in the score in detecting asymptomatic CAN in a cross-sectional setting.