The core secondary metabolome of S. albus, 14 are auxiliary gene clusters and 16 are strain-specific, indicating there is still appreciable chemical diversity to be discovered at the strain level. In step-down Afatinib inhibitory avoidance task, one critical point was the application of various electric current intensities, ranging from 0.3mA to 3mA.The stimulus intensities reflect in the latency of step-down in the test trial. For male Wistar rats, researchers showed the latency for step-down in the test of ±40– 50 seconds with 2 seconds of stimulus in intensities of 0.3mA,0.4mA and 0.5mA ;step-down latencies of 180s in test applied 0.8mA in training and 600 s for 1mA. However, these apparatus did not consider the influence of animal’s bioimpedance on the applied currents. Additionally, several other versions of protocol combine one or two-trials assessments during the task.Therefore, up to now there is no standardized protocol for IAT to properly evaluate aversive memory, principally for fresh researchers in the field. Therefore our aim was to develop and standardize a new device for step-down inhibitory avoidance task, in which the applied electric current has the precise same intensity as the current passing through the animal paws, taking in consideration the animal bioimpedance; the stimulus frequency and intensity are controlled and recorded during the entire experiment. For further tasks, we challenged this new step-down inhibitory avoidance device using a known amnesic drug. The choice of frequency was based in frequencies utilized in electric stimulation machines for physiotherapy proposes using 10–100Hz. The frequency of 10Hz was used by Garin, and the value of frequency in major articles used the inhibitory avoidance apparatus wasn’t specified. These frequencies are controlled by a microcontroller PIC18F4520. The intensity of stimuli varied according to the protocol used and it is specified for voltage received by 100ohms resistor integrated in IAT circuit. This aspect has not been specified by many authors. Indeed, we measured the value of current intensity in the traditional apparatus of Inhibitory Avoidance Task, with a floor containing parallel caliber stainless-steel bars spaced 1 cm apart. So the effective current received by the animals´ paws in traditional apparatus usually is reduced by 21–58% of the selected value when the paws touch the bars.In simple words, a researcher selecting 0.5 mA in the traditional apparatus can be applying 0.25–0.4 mA, but not the choice intensity presenting a high variation in the final results. In our Inhibitory Avoidance apparatus, we increased the contact area of the paws skin and the pair of bars by 0.8 cm² and we decrease the current route, consequently, we drastically decreased the impedance values of paw skin from 1.7 Momhs to 32Komhs. By performing these changes we were able to reduce the decrement of effective current from 21–58% to 0.1–3%. To the electrical shock pass only in the paws and not through the body, in our apparatus, there are three position modes of bars: connected in phase, connected in ground, and off.