LNA array is one of multiple successful examples of microarray technology adopted into miRNA study. Thus, we selected it to represent microarray technology in our analysis. Bead-based hybridization carries an expectation of increased specificity over glass-based microarray. Five-micron polystyrene beads, uniquely colored and covered with oligonucleotide capture probes specific for a single miRNA, are hybridized to biotinylated miRNA in the liquid phase and then they are stained with streptavidin-phycoerythrin. A flow cytometer (+)-JQ1 directs a single column of beads through the path of two lasers; one laser is used to identify the particular miRNA by its bead color, and the other is used to detect bound quantities of miRNA based on the presence of the reporter molecule, phycoerythrin. Bead-based arrays allow for the inclusion of many combinations of miRNA capture beads into a single pool, which are adjusted based on the interaction of bead-coupled probes, and provides greater flexibility over time as miRNA are discovered and corresponding beads are created. Indeed, beads-based miRNA detection is both feasible and attractive for its high speed, heightened accuracy, and relatively low cost. Quantitative real-time RT-PCR assay is a rapid and reproducible methodology with a broad dynamic range compared to Northern blot or conventional RT-PCR when assessing RNA expression. It has been widely applied in miRNA research for years and recognized therein as a gold standard. TaqMan is a relatively mature technology for the qRT-PCR application and has been adopted into miRNA research utilizing a stem-loop structure specific for binding mature miRNA. The development of TaqMan technology has led to an innovative design of Low Density Arrays, a mediumthroughput method for real-time RT-PCR that uses 384-well microfluidics cards. A single TLDA card may assay up to 384 miRNAs. In theory, this technology provides a feasible platform combining miRNA discovery and validation. In this study, we will profile miRNAs from a panel of osteosarcoma xenografts using LNA microarray, beads array, and TLDA, respectively. Systematic comparison and evaluation within and across platforms will be performed. To evaluate the intra-platform reproducibility, we calculated the rank-based Spearman’s correlation coefficients among various miRNA profiles tested on different samples by the same platform. A stable platform is expected to produce similar results across different experiments. In other words, the results from the same sample using the same platform should be reproducible. The Pearson correlation coefficient analysis was banned because the study demonstrated that array profiling data were mostly nonlinear. By using the Spearman’s correlation coefficient measurement to evaluate intra-platform reproducibility, which adopts the rank information, the different scales used in each platform may be ignored and log-transformation can be avoided.