Syn affect its biochemical properties, we sought to recapitulate the modulation of conformation in immortalized cell lines, which would enable us to achieve higher transfection efficiencies required for biochemical studies. First, we transfected three cell lines of different origins with the Myc-aSyn-V5 construct. These cells were then treated with dopamine and processed for FLIM. Interestingly, we found that the in the cell lines of neuronal origin dopamine induced a conformational change similar to that observed in primary neuronal cultures. In limited experiments using HEK cells, we did not observe such a conformational change. Notably, different levels of sensitivity seen in six other mutant variants A231G, V364A, M132A, P512A, S505A and V496A points to the fact that even though there is a single pore for proton antiport and drug binding and translocation, yet there are residues which impart selectivity in drug recognition along with those which affect the transport of all the drugs. Another, well studied TA system is relBE, which is induced by stressful conditions that cause growth arrest by allowing the toxin RelE to act freely. RelE causes cleavage of mRNA codons in the ribosomal A site. We happened to find that miR-107 and miR-185 can suppress cell proliferation in two lung cancer cell lines and induced a G1 arrest of the cell cycle. The extent of growth suppression by these miRNAs is similar to that by the tumor suppressive miRNA, let-7. Here we test the model for its predictions about the expression, size, and symmetry of the Carabelli trait in humans. The Carabelli trait emerges from the lingual surface of the protocone and Dinaciclib usually begins to form after the four major cusps of the molar have initiated The trait ranges in expression from a shallow furrow to a cusp with a free apex which rivals the hypocone, one of the molar’s four principal cusps, in size. We predicted that if the patterning cascade model can explain the presence and size of the Carabelli cusp, then: teeth with smaller intercusp distances relative to crown size would be more likely to possess the Carabelli cusp and, teeth with the smallest intercusp distances relative to crown size would possess the largest Carabelli cusps. Gene expression profiling analysis with the transfection of these miRNAs indicated that only miR-107 showed significant enrichment of cell cycle regulators for the downstream effectors. On the other hand, miR-185 did not significantly repress cell cycle regulator as well as let-7, a known cell cycle regulating miRNA. This cleavage is highly codon specific, occurring between the second and third nucleotides. It has been suggested that RelE might not be an endonuclease itself, but rather may enhance the intrinsic cutting action of the ribosome when it pauses in the translation process. The antitoxin RelB is degraded by the Lon protease.