In the present study, we conducted a comprehensive battery of behavioral tests in C57BL/ 6J patDp/+ mice to evaluate other behavioral abnormalities. Moreover, using ex vivo high performance liquid chromatography, we performed quantitative analyses of biogenic amines in brains of adult and young mice to determine the role of brain monoamines in any measured behavioral abnormalities. The specific hypothesis tested was whether there is a relationship between specific behaviors and brain monoamines in patDp/+ mice, and the results suggest that disturbance of serotonergic signaling during WY 14643 PPAR inhibitor development may cause MG132 abnormal behaviors in these mice. Comprehensive quantification of monoamines also revealed increased DA signaling during developmental stages that may be relevant to the behavioral phenotypes of patDp/+ mice. Many of the various functions and molecular pathways of DA in the mature brain have been reported. However, recent studies reported biogenic amines including DA appear early during embryogenesis, before the onset of synaptogenesis, suggesting that they may play important roles in brain development. In vitro studies demonstrated that during brain development DA acts as a promoter and an inhibitor of the number or length of branching neurites. Furthermore, DA signaling at earlier developmental stages may contribute to postnatal neurogenesis and migration of inhibitory interneurons. As such, alterations in DA signaling during development might also result in the behavioral and neurochemical abnormalities in patDp/+ mice. In this study, we quantified tissue monoamine levels. Future studies should quantify extracellular monoamine levels using microdialysis procedures, and determine whether these abnormalities result from alterations in neurotransmitter release, reuptake, or synthesis. Furthermore, a precise analysis at a nuclear level, such as the raphe nucleus, would be informative. Such a precise study on 5-HT and DA levels at the neural circuit level would be necessary to decipher the neurochemical basis of abnormal behaviors in patDp/+ mice. Additionally, investigations of neurochemistry during embryonic development remain important for understanding when abnormalities of the 5-HT or DA pathways begin. Mice were then gently pulled backward by the tail with their posture parallel to the surface of the table until they released the grid. The peak forelimb grip force applied by the mice was recorded in Newtons. Each mouse was tested three times and the greatest value measured was used for statistical analysis. In the wire hang test, mice were placed on a wire mesh that was then inverted and waved gently, so that the subject gripped the wire. Latency to fall within 60 sec was recorded by counting manually.